This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
Councils blamed the delay on demand for new specialist vehicles, as well as issues with funding despite more than £340m in grants from Defra. You can find out what is happening with your local council's collections further down in this story.
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Фото: Евгений Биятов / РИА Новости
But what followed after spades hit the ground was a string of delays and last-minute cancellations.